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How many times are people given a second chance to redeem themselves?Learn more about how Oath collects and uses data and how our partners collect and use data.Select ' OK' to allow Oath and our partners to use your data, or ' Manage options' to review our partners and your choices.Feces collection Transfer the spin swab into a reaction tube and carefully cut off the stick with the sterile scissors Figure 4C. Prepare a negative control in the same way using an empty medium vial prepared at the same day as the used medium.Open vial and carefully collect the feces with the soaked spin swab Figure 4A. Vortex vortex adapter the samples at highest speed for 10 min Figure 5E. Check all fly strains for a Wolbachia infection before performing your experiments.