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Consequently, an instrument with low stray light is required for the quantitation of high-concentration unknown samples (with high absorbance) using a calibration curve created with standard samples.
The presence of stray light causes distortion in the calibration curve.
Alternatively, a sample with 0.01 % transmittance (4 Abs) appears to have a transmittance of 0.02 % (3.6990 Abs).
That is, errors of 0.0043 Abs and 0.3010 Abs, respectively, occur.
For example, an aqueous solution of Nal does not transmit light at 220 nm.
Initially, the transmittance is measured with a shutter block that completely shuts out all light mounted in the sample compartment (transmittance X).